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Báo cáo y học: "Circulating angiopoietin-1 and angiopoietin-2 in critically ill patients: development and clinical application of two new immunoassays"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Circulating angiopoietin-1 and angiopoietin-2 in critically ill patients: development and clinical application of two new immunoassays. | Available online http ccforum.eom content 1 2 4 R94 Research Circulating angiopoietin-1 and angiopoietin-2 in critically ill patients development and clinical application of two new immunoassays Alexander Lukasz1 Julian Hellpap1 Rudiger Horn2 Jan T Kielstein1 Sascha David1 Hermann Haller1 and Philipp Kumpers1 Department of Nephrology Hannover Medical School Carl-Neuberg-StraBe 1 30625 Hannover Germany department of Gastroenterology Hepatology and Endocrinology Hannover Medical School Carl-Neuberg-StraBe 1 Hannover 30625 Germany Corresponding author Philipp Kumpers kuempers.philipp@mh-hannover.de Received 9 Jun 2008 Revisions requested 4 Jul 2008 Revisions received 9 Jul 2008 Accepted 29 Jul 2008 Published 29 Jul 2008 Critical Care 2008 12 R94 doi 10.1186 cc6966 This article is online at http ccforum.com content 12 4 R94 2008 Lukasz et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Open Access Abstract Introduction In critically ill patients the massive release of angiopoietin-2 Ang-2 from endothelial Weibel-Palade bodies interferes with constitutive angiopoietin-1 Ang-1 Tie2 signaling in endothelial cells thus leading to vascular barrier breakdown followed by leukocyte transmigration and capillary leakage. The use of circulating Ang-1 and Ang-2 as novel biomarkers of endothelial integrity has therefore gained much attention. The preclinical characteristics and clinical applicability of angiopoietin immunoassays however remain elusive. Methods We developed sandwich immunoassays for human Ang-1 immunoradiometric sandwich assay immunoluminometric sandwich assay and Ang-2 ELISA assessed preanalytic characteristics and determined circulating Ang-1 and Ang-2 concentrations in 30 healthy control individuals and in 94 .