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Báo cáo y học: "The human allergens of mesquite (Prosopis juliflora)"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: The human allergens of mesquite (Prosopis juliflora). | Clinical and Molecular Allergy BioMed Central Research The human allergens of mesquite Prosopis juliflora Sue Killian 1 2 and John McMichael1 Open Access Address 1Dept. Research The Institute for Therapeutic Discovery P.O. Box 127 Delanson New York 12053 USA and 2The Institute for Therapeutic Discovery 628 Kerns Mt. Ln. New Market VA 22844 USA Email Sue Killian - skillian@shentel.net John McMichael - titd@shentel.net Corresponding author Published 05 July 2004 Received 23 April 2004 Clinical and Molecular Allergy 2004 2 8 doi l0.ll 86 1476-7961-2-8 Accepted 05 July 2004 This article is available from http www.clinicalmolecularallergy.cOm content 2 1 8 2004 Killian and McMichael licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background A computerized statistical analysis of allergy skin test results correlating patient reactivities initiated our interest in the cross-reactive allergens of mesquite tree pollen. In-vitro testing with mesquite-sensitized rabbits and a variety of deciduous tree pollens revealed so many cross-reactivities that it became apparent there could be more allergens in mesquite than previously described in the world literature. Our purpose was to examine the allergens of mesquite tree pollen Prosopis juliflora which elicit an IgE response in allergic humans so that future research could determine if these human allergens cross-react with various tree pollens in the same manner as did the mesquite antiserum from sensitized rabbits. Methods Proteins from commercial mesquite tree pollen were separated by polyacrylamide gel electrophoresis in the presence of sodium-dodecyl-sulphate. These mesquite proteins were subjected to Western blotting using pooled sera from ten mesquite-sensitive patients and goat antihuman IgE. The allergens were detected using an .