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Báo cáo y học: "DNA signatures for detecting genetic engineering in bacteria"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: DNA signatures for detecting genetic engineering in bacteria. | Open Access Method DNA signatures for detecting genetic engineering in bacteria Jonathan E Allen Shea N Gardner and Tom R Slezak Address Lawrence Livermore National Lab Livermore CA 94550 USA. Correspondence Jonathan E Allen. Email allen99@llnl.gov Published 18 March 2008 Genome Biology 2008 9 R56 doi 10.1 186 gb-2008-9-3-r56 The electronic version of this article is the complete one and can be found online at http genomebiology.com 2008 9 3 R56 Received 23 August 2007 Revised 10 December 2007 Accepted 18 March 2008 2008 Allen et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Using newly designed computational tools we show that despite substantial shared sequences between natural plasmids and artificial vector sequences a robust set of DNA oligomers can be identified that can differentiate artificial vector sequences from all available background viral and bacterial genomes and natural plasmids. We predict that these tools can achieve very high sensitivity and specificity rates for detecting new unsequenced vectors in microarray-based bioassays. Such DNA signatures could be important in detecting genetically engineered bacteria in environmental samples. Background Synthetic vector sequences are of fundamental importance in molecular biology. Cloning and expression vectors are among a multitude of synthetic sequence types commonly used as part of a basic tool set for DNA amplification and protein production 1 . As the emerging maturity of synthetic biology research fast approaches 2 it is reasonable to imagine in the not too distant future the broad-scale manufacture of sophisticated synthetic plasmids to modify existing bacteria and possibly the construction of new functioning synthetic genomes 3 . The .