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Báo cáo sinh học: " A strategy of tumor treatment in mice with doxorubicin-cyclophosphamide combination based on dendritic cell activation by human double-stranded DNA preparation"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: A strategy of tumor treatment in mice with doxorubicin-cyclophosphamide combination based on dendritic cell activation by human double-stranded DNA preparation | Alyamkina et al. Genetic Vaccines and Therapy 2010 8 7 http www.gvt-journal.eom content 8 1 7 GENETIC VACCINES AND THERAPY RESEARCH Open Access A strategy of tumor treatment in mice with doxorubicin-cyclophosphamide combination based on dendritic cell activation by human double-stranded DNA preparation 1.2 2 1.2 1.2 2 Ekaterina A Alyamkina 1 Valeriy P Nikolin Nelly A Popova 1 Evgenia V Dolgova 1 Anastasia S Proskurina Konstantin E Orishchenko2 Yaroslav R Efremov2 Elena R Chernykh3 Alexandr A Ostanin3 Sergey V Sidorov4 Dmitriy M Ponomarenko5 Stanislav N Zagrebelniy1 Sergey S Bogachev2 Mikhail A Shurdov6 Abstract Background Immunization of mice with tumor homogenate after combined treatment with cyclophosphamide CP and double-stranded DNA dsDNA preparation is effective at inhibition of growth of tumor challenged after the treatment. It was assumed that this inhibition might be due to activation of the antigen-presenting cells. The purpose was to develop improved antitumor strategy using mice. We studied the combined action of cytostatics doxorubicin Dox plus CP with subsequent dsDNA preparation on tumor growth. Methods Three-month old CBA Lac mice were used in the experiments. Mice were injected with CP and human dsDNA preparation. The percentage of mature dendritic cells DCs was estimated by staining of mononuclear cells isolated from spleen and bone marrow 3 6 and 9 days later with monoclonal antibodies CD34 CD80 and CD86. In the next set of experiments mice were given intramuscularly injections of 1-3 X 105 tumor cells. Four days later they were injected intravenously with 6-6.7 mg kg Dox and intraperitoneally with 100-200 mg kg CP 200 mkg human DNA was injected intraperitoneally after CP administration. Differences in tumor size between groups were analyzed for statistical significance by Student s t-test. The MTT-test was done to determine the cytotoxic index of mouse leucocytes from treated groups. Results The conducted experiments showed that combined .