Đang chuẩn bị liên kết để tải về tài liệu:
Báo cáo khoa hoc:" A review on SNP and other types of molecular markers and their use in animal genetics"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: A review on SNP and other types of molecular markers and their use in animal genetics | Genet Sei. Evol. 34 2002 275-305 INRA EDP Sciences 2002 DOI 10.1051 gse 2002009 215 Review a review on SNP and other tYpes of molecular markers and their use in animal genetics Alain Vignala Denis Milana Magali SanCristobala André Eggenb a Laboratoire de génétique cellulaire Inra chemin de Borde-Rouge Auzeville BP 27 31326 Castanet-Tolosan cedex France b Laboratoire de génétique biochimique et de cytogénétique Inra domaine de Vilvert 78352 Jouy-en-Josas cedex France Received 11 February 2002 accepted 8 March 2002 Abstract - During the last ten Years the use of molecular markers revealing polymorphism at the DNA level has been playing an increasing part in animal genetics studies. Amongst others the microsatellite DNA marker has been the most widely used due to its easy use by simple PCR followed by a denaturing gel electrophoresis for allele size determination and to the high degree of information provided by its large number of alleles per locus. Despite this a new marker type named SNP for Single Nucleotide Polymorphism is now on the scene and has gained high popularity even though it is only a bi-allelic type of marker. In this review we will discuss the reasons for this apparent step backwards and the pertinence of the use of SNPs in animal genetics in comparison with other marker types. SNP microsatellite molecular marker genome polymorphism 1. introduction older types of molecular genetic markers Molecular markers revealing polymorphisms at the DNA level are now key players in animal genetics. However due to the existence of various molecular biology techniques to produce them and to the various biological implications some can have a large variety exists from which choices will have to be made according to purposes. Two main points have to be considered when using molecular markers for genetic studies. As seen from the molecular biologist s point of view the genotyping procedure should be as simple and have as low a cost as possible in Correspondence and .