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Báo cáo y học: "Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damag"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage. | Yamauchi et al. Genome Biology 2010 11 R66 http genomebiology.eom 2010 11 6 R66 w Genome Biology RESEARCH Open Access Deficiency in mouse Y chromosome long arm gene complement is associated with sperm DNA damage 1 f 1 f 1 2 1 Yasuhiro Yamauchi Jonathan M Riel Zoia Stoytcheva Paul S Burgoyne Monika A Ward Abstract Background Mice with severe non-PAR Y chromosome long arm NPYq deficiencies are infertile in vivo and in vitro. We have previously shown that sperm from these males although having grossly malformed heads were able to fertilize oocytes via intracytoplasmic sperm injection ICSI and yield live offspring. However in continuing ICSI trials we noted a reduced efficiency when cryopreserved sperm were used and with epididymal sperm as compared to testicular sperm. In the present study we tested if NPYq deficiency is associated with sperm DNA damage - a known cause of poor ICSI success. Results We observed that epididymal sperm from mice with severe NPYq deficiency that is deletion of nine-tenths or the entire NPYq gene complement are impaired in oocyte activation ability following ICSI and there is an increased incidence of oocyte arrest and paternal chromosome breaks. Comet assays revealed increased DNA damage in both epididymal and testicular sperm from these mice with epididymal sperm more severely affected. In all mice the level of DNA damage was increased by freezing. Epididymal sperm from mice with severe NPYq deficiencies also suffered from impaired membrane integrity and abnormal chromatin condensation and suboptimal chromatin protamination. It is therefore likely that the increased DNA damage associated with NPYq deficiency is a consequence of disturbed chromatin remodeling. Conclusions This study provides the first evidence of DNA damage in sperm from mice with NPYq deficiencies and indicates that NPYq-encoded gene s may play a role in processes regulating chromatin remodeling and thus in maintaining DNA integrity in sperm. Background The DNA of the .