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Báo cáo y học: "Long noncoding RNA genes: conservation of sequence and brain expression among diverse amniotes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Long noncoding RNA genes: conservation of sequence and brain expression among diverse amniotes. | Chodroff et al. Genome Biology 2010 11 R72 http genomebiology.eom 2010 11 7 R72 Genome Biology RESEARCH Open Access Long noneoding RNA genes conservation of sequence and brain expression among diverse amniotes 1.2 3 1 3 1.3 I- P s z 2 Rebecca A Chodroff1 Leo Goodstadt Tamara M Sirey Peter L Oliver Kay E Davies Eric D Green Zoltán Molnár1 Chris P Ponting1 3 Abstract Background Long considered to be the building block of life it is now apparent that protein is only one of many functional products generated by the eukaryotic genome. Indeed more of the human genome is transcribed into noncoding sequence than into protein-coding sequence. Nevertheless whilst we have developed a deep understanding of the relationships between evolutionary constraint and function for protein-coding sequence little is known about these relationships for non-coding transcribed sequence. This dearth of information is partially attributable to a lack of established non-protein-coding RNA ncRNA orthologs among birds and mammals within sequence and expression databases. Results Here we performed a multi-disciplinary study of four highly conserved and brain-expressed transcripts selected from a list of mouse long intergenic noncoding RNA lncRNA loci that generally show pronounced evolutionary constraint within their putative promoter regions and across exon-intron boundaries. We identify some of the first lncRNA orthologs present in birds chicken marsupial opossum and eutherian mammals mouse and investigate whether they exhibit conservation of brain expression. In contrast to conventional proteincoding genes the sequences transcriptional start sites exon structures and lengths for these non-coding genes are all highly variable. Conclusions The biological relevance of lncRNAs would be highly questionable if they were limited to closely related phyla. Instead their preservation across diverse amniotes their apparent conservation in exon structure and similarities in their pattern of brain .