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Báo cáo y học: "Immunohistochemical localization of mu opioid receptor in the marginal division with comparison to patches in the neostriatum of the rat brain"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Immunohistochemical localization of mu opioid receptor in the marginal division with comparison to patches in the neostriatum of the rat brain | Wang et al. Journal of Biomedical Science 2011 18 34 http www.jbiomedsci.eom content 18 1 34 tì.NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Immunohistochemical localization of mu opioid receptor in the marginal division with comparison to patches in the neostriatum of the rat brain 1 2 1 -3 2 1 4 Chuanxing Wang Si Yun Shu Zhouyi Guo Ye Feng Cai Xinmin Bao Changchun Zeng Bingyi Wu Ziyou Hu4 and Xuemei Liu 1 2 Abstract Background Mu opioid receptor MOR which plays key roles in analgesia and also has effects on learning and memory was reported to distribute abundantly in the patches of the neostriatum. The marginal division MrD of the neostriatum which located at the caudomedial border of the neostriatum was found to stain for enkephalin and substance P immunoreactivities and this region was found to be involved in learning and memory in our previous study. However whether MOR also exists in the MrD has not yet been determined. Methods In this study we used western blot analysis and immunoperoxidase histochemical methods with glucose oxidase-DAB-nickel staining to investigate the expression of MOR in the MrD by comparison to the patches in the neostriatum. Results The results from western blot analyses revealed that the antibody to MOR detected a 53 kDa protein band which corresponded directly to the molecular weight of MOR. Immunohistochemical results showed that punctate MOR-immunoreacted fibers were observed in the patch areas in the rostrodorsal part of the neostriatum but these previous studies showed neither labelled neuronal cell bodies nor were they shown in the caudal part of the neostriatum. Dorsoventrally oriented dark MOR-immunoreactive nerve fibers with individual labelled fusiform cell bodies were firstly observed in the band at the caudomedial border the MrD of the neostriatum. The location of the MOR-immunoreactivity was in the .