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Báo cáo y học: "Identification of 11-amino acid peptides that disrupt Notch-mediated processes in Drosophila"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of 11-amino acid peptides that disrupt Notch-mediated processes in Drosophila | Pi et al. Journal of Biomedical Science 2011 18 42 http www.jbiomedsci.eom content 18 1 42 NSC The cost of publication in Journal of Biomedical Science Is borne by the National Science Council Taiwan JOURNAL OF BIOMEDICAL SCIENCE RESEARCH Open Access Identification of 11-amino acid peptides that disrupt Notch-mediated processes in Drosophila 1 t 11 1 1 1 2 Haiwei Pi Yi-Chun Huang I-Chun Chen Chung-De Lin Hsiao-Fong Yeh and Li-Mei Pai Abstract Background The conserved Notch signaling pathway regulates cell fate decisions and maintains stem cells in multicellular organisms. Up-regulation of Notch signaling is observed in several types of cancer and is causally involved in proliferation and survival of cancer cells. Thus it is of great interest to look for anti-Notch reagents for therapeutic purposes. In model animal Drosophila Notch signaling restricts selection of sensory organ precursors SOPs during external sensory ES organ development. To look for novel genes that can suppress Notch signaling we performed a gain-of-function modifier screen to look for genes that enhance the phenotype of ectopic ES organs induced by overexpression of phyllopod a gene required for SOP specification. Results From the gain-of-function screen we discovered that overexpression of polished rice tarsal-less pri tal increases the numbers of ES organs as well as SOPs. pri tal is a polycistronic gene that contains four short open reading frames encoding three 11-amino acid and one 32-amino acid peptides. Ectopic expression of the 11 amino-acid peptides recapitulates the pri tal misexpression phenotype in ectopic ES organ formation. In situ hybridization experiment reveals that pri tal mRNA is expressed in the SOPs of the chemosensory organs and the stretch-sensing chordotonal organs. In Drosophila wing development the Notch signaling pathway mediates the formation of the dorsal-ventral DV compartmental boundary and the restriction of the vein width from the primordial veins the proveins. We