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Báo cáo y học: "Safety and anti-HIV assessments of natural vaginal cleansing products in an established topical microbicides in vitro testing algorith"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Safety and anti-HIV assessments of natural vaginal cleansing products in an established topical microbicides in vitro testing algorithm. | Lackman-Smith et al. AIDS Research and Therapy 2010 7 22 http www.aidsrestherapy.eom content 7 1 22 AIDS RESEARCH AND THERAPY RESEARCH Open Access Safety and anti-HIV assessments of natural vaginal cleansing products in an established topical microbicides in vitro testing algorithm Carol S Lackman-Smith 1 Beth A Snyder1 Katherine M Marotte1 Mark C Osterling1 Marie K Mankowski1 Maureen Jones1 Lourdes Nieves-Duran1 Nicola Richardson-Harman2 James E Cummins Jr1 3 and Brigitte E Sanders-Beer1 4 Abstract Background At present there is no effective vaccine or other approved product for the prevention of sexually transmitted human immunodeficiency virus type 1 HIV-1 infection. It has been reported that women in resourcepoor communities use vaginally applied citrus juices as topical microbicides. These easily accessible food products have historically been applied to prevent pregnancy and sexually transmitted diseases. The aim of this study was to evaluate the efficacy and cytotoxicity of these substances using an established topical microbicide testing algorithm. Freshly squeezed lemon and lime juice and household vinegar were tested in their original state or in pH neutralized form for efficacy and cytotoxicity in the CCR5-tropic cell-free entry and cell-associated transmission assays CXCR4-tropic entry and fusion assays and in a human PBMC-based anti-HIV-1 assay. These products were also tested for their effect on viability of cervico-vaginal cell lines human cervical explant tissues and beneficial Lactobacillus species. Results Natural lime and lemon juice and household vinegar demonstrated anti-HIV-1 activity and cytotoxicity in transformed cell lines. Neutralization of the products reduced both anti-HIV-1 activity and cytotoxicity resulting in a low therapeutic window for both acidic and neutralized formulations. For the natural juices and vinegar the IC50 was 3.5 0.8-3.5 and the TC50 6.3 1.0-6.3 . All three liquid products inhibited viability of beneficial .