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Báo cáo y học: "tromal Vascular Fraction Transplantation as an Alternative Therapy for Ischemic Heart Failure: Anti-inflammatory Role"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Stromal Vascular Fraction Transplantation as an Alternative Therapy for Ischemic Heart Failure: Anti-inflammatory Role. | Premaratne et al. Journal of Cardiothoracic Surgery 2011 6 43 http www.cardiothoracicsurgery.Org content 6 1 43 JCTS JOURNAL OF CARDIOTHORACIC SURGERY RESEARCH ARTICLE Open Access Stromal Vascular Fraction Transplantation as an Alternative Therapy for Ischemic Heart Failure Anti-inflammatory Role 1 1.2 33 1 1 Goditha U Premaratne Li-Ping Ma Masatoshi Fujita Xue Lin Entela Bollano and Michael Fu Abstract Background The aims of this study were 1 to show the feasibility of using adipose-derived stromal vascular fraction SVF as an alternative to bone marrow mono nuclear cell BM-MNC for cell transplantation into chronic ischemic myocardium and 2 to explore underlying mechanisms with focus on anti-inflammation role of engrafted SVF and BM-MNC post chronic myocardial infarction MI against left ventricular LV remodelling and cardiac dysfunction. Methods Four weeks after left anterior descending coronary artery ligation 32 Male Lewis rats with moderate MI were divided into 3 groups. SVF group n 12 had SVF cell transplantation 6 X 106 cells . BM-MNC group n 12 received BM-MNCs 6 X 106 and the control n 10 had culture medium. At 4 weeks after the final echocardiography histological sections were stained with Styrus red and immunohistochemical staining was performed for a-smooth muscle actin von Willebrand factor CD3 CD8 and CD20. Results At 4 weeks in SVF and BM-MNC groups LV diastolic dimension and LV systolic dimension were smaller and fractional shortening was increased in echocardiography compared to control group. Histology revealed highest vascular density CD3 and CD20 cells in SVF transplanted group. SVF transplantation decreased myocardial mRNA expression of inflammatory cytokines TNF-a IL-6 MMP-1 TIMP-1 and inhibited collagen deposition. Conclusions Transplantation of adipose derived SVF cells might be a useful therapeutic option for angiogenesis in chronic ischemic heart disease. Anti-inflammation role for SVF and BM transplantation might partly benefit for the .