Đang chuẩn bị liên kết để tải về tài liệu:
báo cáo khoa học: "CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway"

Không đóng trình duyệt đến khi xuất hiện nút TẢI XUỐNG

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway | Zheng et al. Journal of Experimental Clinical Cancer Research 2011 30 48 http www.jeccr.eom content 30 1 48 RESEARCH Journal of Experimental Clinical Cancer Research Open Access CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway Jianfeng Zheng1t Rongquan Fu2t Jing Li3 and Xiaozhong Wang1 Abstract Objective To explore the potential role of CpG motif-containing oligonucleotides CpG-ODN in modulating the expression of FasL in HepG2 and Fas in Jurkat cells in vitro and to examine the effect of CpG-ODN treatment on the HepG2 cells-mediated Jurkat cell apoptosis in vitro. Methods The expressions of FasL in HepG2 and Fas in Jurkat cells were examined by real time PCR and flow cytometry FCM . HepG2 and Jurkat cells were co-cultured and the frequency of apoptotic Jurkat cells and levels of activated caspase-3 were determined by FCM. Results Treatment with CpG-ODN down-regulated the expression of FasL in HepG2 cells in a dose- and timedependent manner. In addition treatment with CpG-ODN down-regulated the Fas mRNA transcription and protein expression in Jurkat cells. Treatment of HepG2 cells or Jurkat cells with FasL-neutralizing antibody NOK-2 remarkably inhibited the HepG2-medaited Jurkat cell apoptosis. Pre-treatment of HepG2 or Jurkat cells with CpG-ODN significantly reduced the frequency of HepG2-mediated apoptotic Jurkat cells and inhibited the activation of caspase-3 in Jurkat cells in vitro. Conclusions Our data indicated that treatment with CpG-ODN inhibited the HepG2 cells-mediated Jurkat cell apoptosis by modulating the Fas FasL pathway. Apparently CpG-ODN treatment may be a potential therapeutic reagent for HCC. Keywords CpG-ODN hepatocellular carcinoma apoptosis Introduction Tumors escape immune surveillance through multiple mechanisms. For example tumors can produce inhibitory factors such as transforming growth factor-b TGF-b and vascular endothelial growth factor VEGF leading to the reduced dendritic cell

TÀI LIỆU LIÊN QUAN

báo cáo khoa học: "CpG oligonucleotides suppress HepG2 cells-induced Jurkat cell apoptosis via the Fas-FasL-mediated pathway"

Báo cáo khoa học: "Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens"

Báo cáo y học: " Intratracheal synthetic CpG oligodeoxynucleotide causes acute lung injury with systemic inflammatory response"

Báo cáo y học: " CpG oligonucleotide activates Toll-like receptor 9 and causes lung inflammation in vivo"

Báo cáo y học: "CpG DNA modulates interleukin 1β-induced interleukin-8 expression in human bronchial epithelial (16HBE14o-) cells"

Báo cáo y học: " Local therapy with CpG motifs in a murine model of allergic airway inflammation in IFN-β knock-out mice"

Báo cáo y học: "CpG Immunotherapy in Chenopodium album sensitized mice: The comparison of IFN-gamma, IL-10 and IgE responses in intranasal and subcutaneous administrations"

Báo cáo y học: " Contrasting chromatin organization of CpG islands and exons in the human genome"

Báo cáo y học: "CpG oligodeoxyribonucleotides protect mice from Burkholderia pseudomallei but not Francisella tularensis Schu S4 aerosol''

Báo cáo y học: "The effect of CpG-ODN on antigen presenting cells of the foal"
Đã phát hiện trình chặn quảng cáo AdBlock
Trang web này phụ thuộc vào doanh thu từ số lần hiển thị quảng cáo để tồn tại. Vui lòng tắt trình chặn quảng cáo của bạn hoặc tạm dừng tính năng chặn quảng cáo cho trang web này.