Bệnh nhân được coi là không bị nhiễm khi không có spp Candida. được tìm thấy trong bất kỳ nền văn hóa (máu cũng như nền văn hóa khác) và không có nguy cơ yếu tố cho candidaemia đã có mặt. Một mẫu ml được lấy từ các nền văn hóa máu của những bệnh nhân này và xử lý | Chapter 3 Uninfected patients. Patients were considered not infected when no Candida spp. were found in any culture blood- as well as other cultures and no risk-factors for candidaemia were present. One ml samples were taken from blood cultures of these patients and processed as described. Ten aerobic and ten anaerobic blood cultures derived from five different patients were examined. None of the NASBA-products hybridized with any of the Candida-probes. Patients colonized with Candida spp. Patients were considered colonized when blood cultures were negative for yeast but Candida spp. were cultured from another site. Ten aerobic and ten anaerobic blood cultures derived from five different patients were examined. Candida spp. were cultured from sputum patients 1 2 3 and 5 catheter tip patients 1 2 and 5 urine patients 1 and 4 throat swab anal swab and wound fluid patient 1 and bronchial lavage and aspirate of the sinus cavity patient 5 . One NASBA-product derived from an anaerobic blood culture bottle from patient 4 hybridized with probe 2176. Patients with a culture-proven candidaemia. Ten different patients with at least one blood culture positive for Candida spp. were included. Nine patients were hospitalized in a university hospital one patient was hospitalized in a children s hospital. One ml samples were taken from positive as well as negative blood cultures and processed as described. Patient information and the results of BactT Alert monitoring and the NASBA-assay are depicted in Table 2. All samples that were BacT Alert-positive were also positive in the NASBA-assay. In patients 1 2 and 3 yeast RNA was detected in cultures that remained negative in the BacT Alert monitoring system. In 9 of these 12 BacT Alert-negative NASBA-positives this result was confirmed when the same Candida spp. was isolated after subculturing the blood culture bottles data not shown . Table 3 shows the time-course over which the blood cultures of patients 1 2 and 3 were drawn. .
