Phân tích hiện đại cũng ghi lại chiều rộng di động phân phối màu đỏ (tế bào khối lượng phân phối). Trong hình thái hồng cầu bình thường, điều này tương quan với các đường cong Giá-Jones cho việc phân phối có đường kính di động. Sự khác biệt được sử dụng diagnostically và cho biết sự hiện diện của microspherocytes (tế bào nhỏ hơn với xanh xao trung tâm nhẹ hơn). | Procedures Assays and Normal Values 11 MCHC is determined using this formula MCHC g dl Hemoglobin concentration g dl Hematocrit l l Red Cell Distribution Width RDW Modern analyzers also record the red cell distribution width cell volume distribution . In normal erythrocyte morphology this correlates with the Price-Jones curve for the cell diameter distribution. Discrepancies are used diagnostically and indicate the presence of microspherocytes smaller cells with lighter central pallor . Reticulocyte Count Reticulocytes can be counted using flow cytometry and is based on the light absorbed by stained aggregates of reticulocyte organelles. The data are recorded as the number of reticulocytes per mill o of the total number of erythrocytes. Reticulocytes can of course be counted in a counting chamber using a microscope. While this method is not particularly laborious it is mostly employed in laboratories that often deal with or have a special interest in anemia. Reticulocytes are young erythrocytes immediately after they have extruded their nuclei they contain as a remainder of aggregated cell organelles a net-like structure hence the name reticulocyte that is not discernible after the usual staining procedures for leukocytes but can be observed after vital staining of cells with brilliant cresyl blue or new methylene blue. The staining solution is mixed in an Eppendorf tube with an equal volume of EDTA blood and incubated for 30 minutes. After repeated mixing a blood smear is prepared and allowed to dry. The sample is viewed using a microscope equipped with an oil immersion lens. The ratio of reticulocytes to erythrocytes is determined and plotted as reticulocytes per 1000 erythrocytes per mill . Normal values are listed in Table 2 p. 12. Theml Color Atlas of Hematology 2004 Thieme All rights reserved. Usage subject to terms and conditions of license. Theml Color Atlas of Hematology 2004 Thieme All rights reserved. Usage subject to terms and conditions of license. .
