Các đối tượng của Truyền dịch tế bào tiền thân tạo máu allogeneic là thiết lập một ghép thường trực của các tế bào nguyên bản truyền người nhận. Số phận của các tế bào nguyên bản allogeneic truyền vào tĩnh mạch lưu thông phụ thuộc vào khả năng của mình để lưu lượng truy cập đến các trang web của khoan dung tạo máu ('vi môi trường') và quản lý hai hiện tượng miễn dịch | CHAPTER 14 Transfusion of haematopoietic cells The object of transfusing allogeneic haematopoietic progenitor cells is to establish a permanent graft of transfused progenitor cells in the recipient. The fate of allogeneic progenitor cells infused into the venous circulation depends on their ability to traffic to sites of haematopoietic tolerance microenvironment and on managing two immunological phenomena 1 the rejection of donor progenitor cells by the host immunological response and 2 an immunological reaction of grafted immunologically competent cells against the host GvHD. Both of these reactions depend on the degree of histocompatibility between donor and recipient and also on the immunological competence of the recipient. Engraftment and kinetics also depend on patient age disease status the preparative regimen GvHD prophylaxis and the cellular content of the graft. Bone marrow was the original source of progenitor cells for haematopoietic grafting but mobilized peripheral blood and cord blood have gradually supplanted marrow as a source of PC. The engraftment potential of the component is commonly designated in terms of mononuclear cells that express the CD34 antigen the cluster designation of a transmembrane glycoprotein present on haematopoietic progenitor cells Krause et al. 1996 although accessory cells in the graft clearly play an important role Ash et al. 1991 . Cells that express CD34 include lineage-committed haematopoietic progenitors multipotent progenitors and possibly pluripotent stem cells as well. Flow cytometric assays are used to quantify CD34 cells in both the donor and the component. However problems with interlaboratory accuracy and reproducibility especially of different PC sources have been notorious even with the adoption of a standardized technique Sutherland et al. 1996 Keeney et al. 1998 . Peripheral blood-derived progenitor cells Peripheral blood-derived progenitor cells PBPCs were reported to circulate in mammalian blood as early .
