Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:CD7 in acute myeloid leukemia: correlation with loss of wild-type CEBPA, consequence of epigenetic regulation | Rohrs et al. Journal of Hematology Oncology 2010 3 15 http content 3 1 15 JOURNAL OF HEMATOLOGY ONCOLOGY RESEARCH Open Access CD7 in acute myeloid leukemia correlation with loss of wild-type CEBPA consequence of epigenetic regulation Sonja Rohrs 1 Michaela Scherr2 Julia Romani1 Margarete Zaborski1 Hans G Drexler1 and Hilmar Quentmeier 1 Abstract Background CD7 is a negative prognostic marker in myeloid malignancies. In acute myeloid leukemia AML an inverse correlation exists between expression of wild-type CEBPA and CD7. Aim of this study was to find out whether C EBPa is a negative regulator of CD7 and which other regulatory mechanisms might be involved. Results As already described for primary AML cells the majority of AML cell lines tested were either C EBPa CD7- or C EBPa- CD7 . However the existence of isolated CD7 cell lines expressing wild-type C EBPa challenges the notion that C EBPa acts as a unique repressor of CD7. Furthermore ectopic expression of CEBPA did not reduce CD7in CD7 cells and knock-down of C EBPa failed to induce CD7 in CD7- cells. In contrast the DNA demethylating agent Aza-2 deoxycytidine triggered CD7 expression in CD7- AML and in T-cell lines suggesting epigenetic regulation of CD7. Bisulfite sequencing data confirmed that CpGs in the CD7 exon1 region are methylated in CD7- cell lines and unmethylated in CD7 cell lines. Conclusion We confirmed an inverse correlation between the expression of wild-type CEBPA and of CD7 in AML cells. Our results contradict the hypothesis that C EBPa acts as repressor for CD7 and instead show that epigenetic mechanisms are responsible for CD7 regulation in AML cells as well as in T-cells the typical CD7 expressing cell type. Background CCAAT enhancer binding factor alpha CEBPA located on chromosome encodes a transcription factor that is of importance for granulocytic differentiation 1 . C EBPa is upregulated during myelomonocytic development and positively affects expression of .
