Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A nano-view of West Nile virus-induced cellular changes during infection | Journal of Nanobiotechnology BioMed Central Research Open Access A nano-view of West Nile virus-induced cellular changes during infection Jason WM Lee and Mah-Lee Ng Address Department of Microbiology 5 Science Drive 2 National University of Singapore Singapore 117597 Singapore Email Jason WM Lee - g0300962@ Mah-Lee Ng - micngml@ Corresponding author Published 29 June 2004 Received 05 April 2004 Journal of Nanobiotechnology 2004 2 6 doi 1477-3 155-2-6 Accepted 29 June 2004 This article is available from http content 2 1 6 2004 Lee and Ng licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background Microscopic imaging of viruses and their interactions with and effects on host cells are frequently held back by limitations of the microscope s resolution or the invasive nature of the sample preparation procedures. It is also difficult to have a technique that would allow simultaneous imaging of both surface and sub-surface on the same cell. This has hampered endeavours to elucidate virus-host interactions. Atomic Force Microscopy AFM which is commonly used in the physical sciences is now becoming a good correlative form of microscopy used to complement existing optical confocal and electron microscopy for biological applications Results In this study the West Nile Sarafend virus-infected Vero cell model was used. The atomic force microscope was found to be useful in producing high resolution images of virus-host events with minimal sample processing requirements. The AFM was able to image the budding of the West Nile Sarafend virus at the infected cell surface. Proliferation of the filopodia and thickening of clusters of actin filaments accompanied West Nile virus replication. Conclusions The study shows that the AFM is useful for
