Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization | Journal of Nanobiotechnology BioMed Central Short Communication A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization Kensuke Kojima Tomoyuki Kaneko and Kenji Yasuda Open Access Address Department of Life Sciences Graduate school of Arts and Sciences University of Tokyo 3-8-1 Komaba Meguro Tokyo 153-8902 JAPAN Email Kensuke Kojima - mhendrix@ Tomoyuki Kaneko - ckaneko@ Kenji Yasuda - cyasuda@ Corresponding author Published 09 September 2004 Received 20 December 2003 Accepted 09 September 2004 Journal of Nanobiotechnology 2004 2 9 doi 1477-3 155-2-9 This article is available from http content 2 1 9 2004 Kojima et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have developed a new method that enables agar microstructures to be used to cultivate cardiac myocyte cells in a manner that allows their connection patterns to be controlled. Non-contact three-dimensional photo-thermal etching with a 1064-nm infrared focused laser beam was used to form the shapes of agar microstructures. This wavelength was selected as it is not absorbed by water or agar. Identical rat cardiac myocytes were cultured in adjacent microstructures connected by microchannels and the interactions of asynchronous beating cardiac myocyte cells observed. Two isolated and independently beating cardiac myocytes were shown to form contacts through the narrow microchannels and by 90 minutes had synchronized their oscillations. This occurred by one of the two cells stopping their oscillation and following the pattern of the other cell. In contrast when two sets of synchronized beating cells came into
