Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Rapid self-assembly of DNA on a microfluidic chip | Journal of Nanobiotechnology BioMed Central Research Rapid self-assembly of DNA on a microfluidic chip Yao Zheng1 Tim Footz2 Dammika P Manage1 and Christopher James Backhouse 1 Open Access Address department of Electrical and Computer Engineering 2nd Floor ECERF Building 9107 - 116St. University of Alberta Edmonton Alberta T6G 2V4 Canada and department of Medical Genetics University of Alberta Edmonton Alberta Canada Email Yao Zheng - zheng@ Tim Footz - tfootz@ Dammika P Manage - manage@ ChristopherJames Backhouse - chrisb@ Corresponding author Published 18 February 2005 Received 21 July 2004 Accepted 18 February 2005 Journal of Nanobiotechnology 2005 3 2 doi 1477-3 155-3-2 This article is available from http content 3 1 2 2005 Zheng et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background DNA self-assembly methods have played a major role in enabling methods for acquiring genetic information without having to resort to sequencing a relatively slow and costly procedure. However even self-assembly processes tend to be very slow when they rely upon diffusion on a large scale. Miniaturisation and integration therefore hold the promise of greatly increasing this speed of operation. Results We have developed a rapid method for implementing the self-assembly of DNA within a microfluidic system by electrically extracting the DNA from an environment containing an uncharged denaturant. By controlling the parameters of the electrophoretic extraction and subsequent analysis of the DNA we are able to control when the hybridisation occurs as well as the degree of hybridisation. By avoiding off-chip processing or long thermal treatments we
