Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Direct microcontact printing of oligonucleotides for biochip applications | Journal of Nanobiotechnology BioMed Central Research Open Access Direct microcontact printing of oligonucleotides for biochip applications C Thibault 1 V Le Berre 2 3 S Casimirius1 E Trévisiol2 3 J Francois 2 3 and C Vieu 1 Address 1LAAS-CNRS 7 avenue du Colonel Roche 31077 TOULOUSE Cedex 4 2Biochips Platform Genopole Toulouse UMR-CNRS 5504 INRA 792 135 avenue de Rangueil 31077 TOULOUSE Cedex 4 and 3Laboratoire de Biotechnologie Bioprocédés UMR-CNRS 5504 INRA 792 135 avenue de Rangueil 31077 TOULOUSE Cedex 4 Email C Thibault - cthibaul@ V Le Berre - leberre@ S Casimirius - scasimir@ E Trévisiol - trevisiol@ J Francois - fran_jm@ C Vieu - vieu@ Corresponding authors fEqual contributors Published 01 July 2005 Received 11 April 2005 Journal of Nanobiotechnology 2005 3 7 doi 1477-3155-3-7 Accepted 01 July 2005 This article is available from http content 3 1 7 2005 Thibault et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background A critical step in the fabrication of biochips is the controlled placement of probes molecules on solid surfaces. This is currently performed by sequential deposition of probes on a target surface with split or solid pins. In this article we present a cost-effective procedure namely microcontact printing using stamps for a parallel deposition of probes applicable for manufacturing biochips. Results Contrary to a previous work we showed that the stamps tailored with an elastomeric poly dimethylsiloxane material did not require any surface modification to be able to adsorb oligonucleotides or PCR products. The adsorbed DNA molecules are subsequently printed efficiently on a target .
