Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Atomic force microscopy: a powerful tool for high-resolution imaging of spermatozoa | Journal of Nanobiotechnology BioMed Central Review Open Access Atomic force microscopy a powerful tool for high-resolution imaging of spermatozoa Sunil Kumar1 Koel Chaudhury 1 Prasenjit Sen2 and Sujoy K Guha1 Address 1School of Medical Science and Technology Indian Institute of Technology Kharagpur 721 302 India and 2School of Physical Sciences Jawaharlal Nehru University New Delhi-110067 India Email Sunil Kumar - sunilkumar1@ Koel Chaudhury - koel@ Prasenjit Sen - psen0700@ Sujoy K Guha - guha_sk@ Corresponding author Published 27 September 2005 Received 05 April 2005 Accepted 27 September 2005 Journal of Nanobiotechnology 2005 3 9 doi 1477-3 155-3-9 This article is available from http content 3 1 9 2005 Kumar et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Atomic force microscopy AFM has emerged as the only technique capable of real-time imaging of the surface of a living cell at nano-resolution. Since AFM provides the advantage of directly observing living biological cells in their native environment this technique has found many applications in pharmacology biotechnology microbiology structural and molecular biology genetics and other biology-related fields. AFM has also proved to be a valuable tool for reproductive biologists. An exhaustive review on the various applications of AFM to sperm cells is presented. AFM has been extensively applied for determining the structural and topological features of spermatozoa. Unstained unfixed spermatozoa in their natural physiological surroundings can be imaged by this technique which provides valuable information about the morphological and pathological defects in sperm cells as
