Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Inorganic phosphate nanorods are a novel fluorescent label in cell biology | Journal of Nanobiotechnology BioMed Central Research Open Access Inorganic phosphate nanorods are a novel fluorescent label in cell biology Chitta Ranjan Patra Resham Bhattacharya Sujata Patra Sujit Basu Priyabrata Mukherjee and Debabrata Mukhopadhyay Address Department of Biochemistry and Molecular Biology Mayo Clinic Cancer Center Mayo Clinic Rochester Minnesota USA Email Chitta Ranjan Patra - Resham Bhattacharya - Sujata Patra - Sujit Basu - Priyabrata Mukherjee - Debabrata Mukhopadhyay - Corresponding author Published 30 October 2006 Received 28 July 2006 Accepted 30 October 2006 Journal of Nanobiotechnology 2006 4 11 doi 1477-3155-4-1 1 r This article is available from http content 4 1 11 2006 Patra et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We report the first use of inorganic fluorescent lanthanide europium and terbium ortho phosphate LnPO4 H2O Ln Eu and Tb nanorods as a novel fluorescent label in cell biology. These nanorods synthesized by the microwave technique retain their fluorescent properties after internalization into human umbilical vein endothelial cells HUVEC 786-O cells or renal carcinoma cells RCC . The cellular internalization of these nanorods and their fluorescence properties were characterized by fluorescence spectroscopy FS differential interference contrast DIC microscopy confocal microscopy and transmission electron microscopy TEM . At concentrations up to 50 pg ml the use of 3H -thymidine incorporation assays apoptosis assays TUNEL and trypan blue exclusion illustrated the non-toxic .
