Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles | Journal of Nanobiotechnology BioMed Central Open Access Truncated forms of viral VP2 proteins fused to EGFP assemble into fluorescent parvovirus-like particles Leona Gilbert Jouni Toivolat Outi Vălilehto Taija Saloniemi Claire Cunningham Daniel White Anna R Măkelă Eila Korhonen Matti Vuento and Christian Oker-Blom Address Department of Biological and Environmental Science and Nanoscience Center . Box 35 40014 University of Jyvăskylă Finland Email Leona Gilbert - lgilbert7@ Jouni Toivola - jopato@ Outi Vălilehto - outvali@ Taija Saloniemi - tsalon@ Claire Cunningham - Daniel White - dan@ Anna R Măkelă - anrimake@ Eila Korhonen - eikorhon@ Matti Vuento - vuento@ Christian Oker-Blom - okerblom@ Corresponding author fEqual contributors Published 08 December 2006 Received 31 March 2006 J . rr . Accepted 08 December 2006 Journal of Nanobiotechnology 2006 4 13 doi l477-3l55-4-l 3 This article is available from http content 4 l l3 2006 Gilbert et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Fluorescence correlation spectroscopy FCS monitors random movements of fluorescent molecules in solution giving information about the number and the size of for example nanoparticles. The canine parvovirus VP2 structural protein as well as N-terminal deletion mutants of VP2 -l4 -23 and -40 amino acids were fused to the C-terminus of the enhanced green fluorescent protein EGFP . The proteins were produced in insect cells purified and analyzed by western blotting confocal and electron microscopy as
