Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization | Journal of Nanobiotechnology BioMed Central Research Open Access mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization Hironori Uehara Yuji Kunitomi Atsushi Ikai and Toshiya Osada Address Department of Life Science Graduate School of Bioscience and Biotechnology Tokyo Institute of Technology Nagatsuta Midori-ku Yokohama 226-8501 Japan Email Hironori Uehara - huehara@ Yuji Kunitomi - Atsushi Ikai - Toshiya Osada - Corresponding author Published 10 October 2007 Received 23 May 2007 _k r-7 iirtz n-7-7- irrr-7 Accepted 10 October 2007 Journal of Nanobiotechnology 2007 5 7 doi 1477-3 155-5-7 r This article is available from http content 5 1 7 2007 Uehara et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The localization of specific mRNA generates cell polarity by controlling the translation sites of specific proteins. Although most of these events depend on differences in gene expression no method is available to examine time dependent gene expression of individual living cells. In situ hybridization ISH is a powerful and useful method for detecting the localization of mRNAs but it does not allow a time dependent analysis of mRNA expression in single living cells because the cells have to be fixed for mRNA detection. To overcome these issues the extraction of biomolecules such as mRNAs proteins and lipids from living cells should be performed without severe damage to the cells. In previous studies we have reported a single cell nanoprobe SCN method to examine gene expression of individual living cells using atomic .
