Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay | Journal of Nanobiotechnology BioMed Central Research Capillary electrophoresis for the characterization of quantum dots after non-selective or selective bioconjugation with antibodies for immunoassay Mark Pereira and Edward PC Lai Open Access Address Department of Chemistry Ottawa-Carleton Chemistry Institute Carleton University Ottawa ON K1S 5B6 Canada Email Mark Pereira - mpereir2@ Edward PC Lai - edward_lai@ Corresponding author Published I October 2008 Received 3 May 2008 J. rr Accepted 1 October 2008 Journal of Nanobiotechnology 2008 6 10 doi 186 1477-3155-6-10 This article is available from http content 6 1 10 2008 Pereira and Lai licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Capillary electrophoresis coupled with laser-induced fluorescence was used for the characterization of quantum dots and their conjugates to biological molecules. The CE-LIF was laboratory-built and capable of injection hydrodynamic and electrokinetic from sample volumes as low as 4 pL via the use of a modified micro-fluidic chip platform. Commercially available quantum dots were bioconjugated to proteins and immunoglobulins through the use of established techniques non-selective and selective . Non-selective techniques involved the use of EDCHCl sulfo-NHS for the conjugation of BSA and myoglobin to carboxylic acid-functionalized quantum dots. Selective techniques involved 1 the use of heterobifunctional crosslinker sulfo-SMCC for the conjugation of partially reduced IgG to amine-functionalized quantum dots and 2 the conjugation of periodate-oxidized IgGs to hydrazide-functionalized quantum dots. The migration times of these conjugates were determined in comparison to their .
