Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Nanometric self-assembling peptide layers maintain adult hepatocyte phenotype in sandwich cultures | Wu et al. Journal of Nanobiotechnology 2010 8 29 http content 8 1 29 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Nanometric self-assembling peptide layers maintain adult hepatocyte phenotype in sandwich cultures I Ạ I 1 f 11 rl h I s r f Di i Z A2 3f I v r A zd 7 h li I I Z I-2 c It I t z l Z K D Z zzzA r 3 DI Tr D- v I- 4 Jonathan wu Nuria Mari-DUye Teresa rernandez Murnos Salvador Borros Pierro ravia Carlos E Semino1 2 5 Abstract Background Isolated hepatocytes removed from their microenvironment soon lose their hepatospecific functions when cultured. Normally hepatocytes are commonly maintained under limited culture medium supply as well as scaffold thickness. Thus the cells are forced into metabolic stress that degenerate liver specific functions. This study aims to improve hepatospecific activity by creating a platform based on classical collagen sandwich cultures. Results The modified sandwich cultures replace collagen with self-assembling peptide RAD16-I combined with functional peptide motifs such as the integrin-binding sequence RGD and the laminin receptor binding sequence YIG to create a cell-instructive scaffold. In this work we show that a plasma-deposited coating can be used to obtain a peptide layer thickness in the nanometric range which in combination with the incorporation of functional peptide motifs have a positive effect on the expression of adult hepatocyte markers including albumin CYP3A2 and HNF4-alpha. Conclusions This study demonstrates the capacity of sandwich cultures with modified instructive self-assembling peptides to promote cell-matrix interaction and the importance of thinner scaffold layers to overcome mass transfer problems. ve believe that this bioengineered platform improves the existing hepatocyte culture methods to be used for predictive toxicology and eventually for hepatic assist technologies and future artificial organs. Background The liver is an important and complex organ that plays a .
