Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Design and characterization of protein-quercetin bioactive nanoparticles | Fang et al. Journal of Nanobiotechnology 2011 9 19 http content 9 1 19 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Design and characterization of protein-quercetin bioactive nanoparticles 1 1 J I z-1 1 1 2 1 1 1 Ru Fang Hao Jing Zhi Chai Guanghua Zhao Serge Stoll Fazheng Ren Fei Liu and Xiaojing Leng Abstract Background The synthesis of bioactive nanoparticles with precise molecular level control is a major challenge in bionanotechnology. Understanding the nature of the interactions between the active components and transport biomaterials is thus essential for the rational formulation of bio-nanocarriers. The current study presents a single molecule of bovine serum albumin BSA lysozyme Lys or myoglobin Mb used to load hydrophobic drugs such as quercetin Q and other flavonoids. Results Induced by dimethyl sulfoxide DMSO BSA Lys and Mb formed spherical nanocarriers with sizes less than 70 nm. After loading Q the size was further reduced by 30 . The adsorption of Q on protein is mainly hydrophobic and is related to the synergy of Trp residues with the molecular environment of the proteins. Seven Q molecules could be entrapped by one Lys molecule 9 by one Mb and 11 by one BSA. The controlled releasing measurements indicate that these bioactive nanoparticles have long-term antioxidant protection effects on the activity of Q in both acidic and neutral conditions. The antioxidant activity evaluation indicates that the activity of Q is not hindered by the formation of protein nanoparticles. Other flavonoids such as kaempferol and rutin were also investigated. Conclusions BSA exhibits the most remarkable abilities of loading controlled release and antioxidant protection of active drugs indicating that such type of bionanoparticles is very promising in the field of bionanotechnology. Background Over the last several decades the development of nanoparticles as drug delivery systems has gained considerable interest. Nanotoxicology research has
