Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Determination of pore size distribution at the cell-hydrogel interface | Leal-Egana et al. Journal of Nanobiotechnology 2011 9 24 http content 9 1 24 JOURNAL OF NANOBIOTECHNOLOGY SHORT COMMUNICATION Open Access Determination of pore size distribution at the cell-hydrogel interface Aldo Leal-Egana1 Ulf-Dietrich Braumann2 3 Aranzazu Diaz-Cuenca4 5 Marcin Nowicki6 and Augustinus Bader1 Abstract Background Analyses of the pore size distribution in 3D matrices such as the cell-hydrogel interface are very useful when studying changes and modifications produced as a result of cellular growth and proliferation within the matrix as pore size distribution plays an important role in the signaling and microenvironment stimuli imparted to the cells. However the majority of the methods for the assessment of the porosity in biomaterials are not suitable to give quantitative information about the textural properties of these nano-interfaces. Findings Here we report a methodology for determining pore size distribution at the cell-hydrogel interface and the depth of the matrix modified by cell growth by entrapped HepG2 cells in microcapsules made of and w v alginate. The method is based on the estimation of the shortest distance between two points of the fibril-like network hydrogel structures using image analysis of TEM pictures. Values of pore size distribution determined using the presented method and those obtained by nitrogen physisorption measurements were compared showing good agreement. A combination of these methodologies and a study of the cell-hydrogel interface at various cell culture times showed that after three days of culture HepG2 cells growing in hydrogels composed of w v alginate had more coarse of pores at depths up to 40 nm inwards a phenomenon most notable in the first 20 nm from the interface . This coarsening phenomenon was weakly observed in the case of cells cultured in hydrogels composed of w v alginate. Conclusions The method purposed in this paper allows us to obtain information .
