Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation | Nejadmoghaddam et al. Journal of Nanobiotechnology 2011 9 31 http content 9 1 31 JOURNAL OF NANOBIOTECHNOLOGY RESEARCH Open Access Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins helpful hints for the optimized nanomagnetisable matrix preparation Mohammad Reza NejadmoghaddamC Mahmood ChamankhahC Saeed Zarei2 and Amir Hassan Zarnani1 3 Abstract Background Several materials are available in the market that work on the principle of protein magnetic fishing by their histidine His tags. Little information is available on their performance and it is often quoted that greatly improved purification of histidine-tagged proteins from crude extracts could be achieved. While some commercial magnetic matrices could be used successfully for purification of several His-tagged proteins there are some which have been proved to operate just for a few extent of His-tagged proteins. Here we address quantitative evaluation of three commercially available Nickel nanomagnetic beads for purification of two His-tagged proteins expressed in Escherichia coli and present helpful hints for optimized purification of such proteins and preparation of nanomagnetisable matrices. Results Marked differences in the performance of nanomagnetic matrices principally on the basis of their specific binding capacity recovery profile the amount of imidazole needed for protein elution and the extent of target protein loss and purity were obtained. Based on the aforesaid criteria one of these materials featured the best purification results SiMAG N-NTA Nickel for both proteins at the concentration of 4 mg ml while the other two SiMAC-Nickel and SiMAG CS-NTA Nickel did not work well with respect to specific binding capacity and recovery profile. Conclusions Taken together functionality of different types of nanomagnetic matrices vary considerably. This variability may not only be dependent upon the structure and .
