Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Comparison of Radioimmuno and Carbon Nanotube Field-Effect Transistor Assays for Measuring Insulin-Like Growth Factor-1 in a Preclinical Model of Human Breast Cancer | Jones et al. Journal of Nanobiotechnology 2011 9 36 http content 9 1 36 JOURNAL OF NANOBIOTECHNOLOGY SHORT COMMUNICATION Open Access Comparison of Radioimmuno and Carbon Nanotube Field-Effect Transistor Assays for Measuring Insulin-Like Growth Factor-1 in a Preclinical Model of Human Breast Cancer Laundette P Jones1 Steingrimur Stefansson2 Man S Kim2 and Saeyoung N Ahn2 Abstract Background To realize the promise of personalized medicine diagnostic instruments used for detecting and measuring biomarkers must become smaller faster and less expensive. Although most techniques used currently to detect biomarkers are sensitive and specific many suffer from several disadvantages including their complexity high cost and long turnaround time. One strategy to overcome these problems is to exploit carbon nanotube CNT based biosensors which are sensitive use inexpensive disposable components and can be easily adapted to current assay protocols. In this study we investigated the applicability of using a CNT field-effect transistor CNT-FET as a diagnostic instrument for measuring cancer biomarkers in serum using a mouse model of Breast Cancer Susceptibility 1-related breast cancer. Insulin like growth factor-1 IGF-1 was chosen because it is highly relevant in breast cancer and because measuring serum IGF-1 levels by conventional methods is complicated due to specific IGF-1 serum binding proteins. Findings Our results show that there is good correlation between the two platforms with respect to detecting serum IGF-1. In fact the CNT-FETs required only one antibody gave real-time results and required approximately 100-fold less mouse serum than the radioimmunoassay. Conclusions Both IGF-1 radioimmuno and CNT-FET assays gave comparable results. Indeed the CNT-FET assay was simpler and faster than the radioimmunoassay. Additionally the low serum sample required by CNT-FETs can be especially advantageous for studies constricted by limited amount of human .
