báo cáo khoa học: " On-chip constructive cell-network study (II): on-chip quasi-in vivo cardiac toxicity assay for ventricular tachycardia/fibrillation measurement using ring-shaped closed circuit microelectrode with lined-up cardiomyocyte cell network"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: On-chip constructive cell-network study (II): on-chip quasi-in vivo cardiac toxicity assay for ventricular tachycardia/fibrillation measurement using ring-shaped closed circuit microelectrode with lined-up cardiomyocyte cell network | Nomura et al. Journal of Nanobiotechnology 2011 9 39 http content 9 1 39 JOURNAL OF NANOBIOTECHNOLOGY SHORT COMMUNICATION Open Access On-chip constructive cell-network study II on-chip quasi-in vivo cardiac toxicity assay for ventricular tachycardia fibrillation measurement using ring-shaped closed circuit microelectrode with lined-up cardiomyocyte cell network Fumimasa Nomura Tomoyuki Kaneko Akihiro Hattori and Kenji Yasuda Abstract Backgrounds Conventional in vitro approach using human ether-a-go-go related gene hERG assay has been considered worldwide as the first screening assay for cardiac repolarization safety. However it does not always oredict the potential QT prolongation risk or pro-arrhythmic risk correctly. For adaptable preclinical strategiesto evaluate global cardiac safety an on-chip quasi-in vivo cardiac toxicity assay for lethal arrhythmia ventricular tachyarrhythmia measurement using ring-shaped closed circuit microelectrode chip has been developed. Results The ventricular electrocardiogram ECG -like field potential data which includes both the repolarization and the conductance abnormality was acquired from the self-convolutied extracellular field potentials FPs of a lined-up cardiomyocyte network on a circle-shaped microelectrode in an agarose microchamber. When Astemisol applied to the closed-loop cardiomyocyte network self-convoluted FP profile of normal beating changed into an early afterdepolarization EAD like waveform and then showed ventricular tachyarrhythmias and ventricular fibrilations VT Vf . QT-prolongation-like self-convoluted FP duration prolongation and its fluctuation increase was also observed according to the increase of Astemizole concentration. Conclusions The results indicate that the convoluted FPs of the quasi-in vivo cell network assay includes both of the repolarization data and the conductance abnormality of cardiomyocyte networks has the strong potential to prediction lethal arrhythmia. .