Tham khảo luận văn - đề án 'báo cáo y học: "characterization of protein tyrosine phosphatase h1 knockout mice in animal models of local and systemic inflammation"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Patrignani et al. Journal of Inflammation 2010 7 16 http content 7 1 16 JOURNAL OF INFLAMMATION RESEARCH Open Access Characterization of protein tyrosine phosphatase H1 knockout mice in animal models of local and systemic inflammation Claudia Patrignani 1 2 David T Lafont1 2 3 Valeria Muzio1 4 Beatrice Gréco1 5 Rob Hooft van Huijsduijnen6 and Paola F Zaratin1 7 Abstract Background PTPH1 is a protein tyrosine phosphatase expressed in T cells but its effect on immune response is still controversial. PTPH1 dephosphorylates TCRzeta in vitrO inhibiting the downstream inflammatory signaling pathway however no immunological phenotype has been detected in primary T cells derived from PTPH1-KO mice. The aim of the present study is to characterize PTPH1 phenotype in two in vivo inflammatory models and to give insights in possible PTPH1 functions in cytokine release. Methods We challenged PTPH1-KO mice with two potent immunomodulatory molecules carrageenan and LPS in order to determine PTPH1 possible role in inflammatory response in vivo. Cytokine release inflammatory pain and gene expression were investigated in challenged PTPH1-WT and KO mice. Results The present study shows that carrageenan induces a trend of slightly increased spontaneous pain sensitivity in PTPH1-KO mice compared to WT wild-type littermates but no differences in cytokine release induced pain perception and cellular infiltration have been detected between the two genotypes in this mouse model. On the other hand LPS-induced TNFO MCP-1 and IL10 release was significantly reduced in PTPH1-KO plasma compared to WTs 30 and 60 minutes post challenge. No cytokine release modulation was detectable 180 minutes post LPS challenge. Conclusion In conclusion the present study points out a slight potential role for PTPH1 in spontaneous pain sensitivity and it indicates that this phosphatase might play a role in the positive regulation of the LPS-induced cytokines release in vivO in contrast
