Tham khảo luận văn - đề án 'báo cáo y học: "loss of function mutation in toll-like receptor-4 does not offer protection against obesity and insulin resistance induced by a diet high in trans fat in mice"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Vijay-Kumar et al. Journal of Inflammation 2011 8 2 http content 8 1 2 JOURNAL OF INFLAMMATION RESEARCH Open Access Loss of function mutation in toll-like receptor-4 does not offer protection against obesity and insulin resistance induced by a diet high in trans fat in mice 1 1 1 2 1 f -3 Matam Vijay-Kumar Jesse D Aitken Frederic A Carvalho Thomas R Ziegler Andrew T Gewirtz Vijay Ganji Abstract Background Toll-like receptor-4 TLR4 triggers inflammatory signaling in response to microbial lipoploysaccharide. It has been reported that loss ofTLR4 protected against saturated fat-induced inflammation and insulin resistance. It is not known whether loss of TLR4 function offers protection against trans fat TF induced obesity inflammation and insulin resistance. We investigated whether mice with loss of function mutation in TLR4 were resistant to TF-induced pathologies such as obesity inflammation hyperglycemia and hyperinsulinemia. Methods C57BL 6j and C57BL 10 mice were cross bred to generate TLR4 mutant and wild type WT . TLR4 mutant n 12 and WT n 12 mice were fed either low fat LF fat energy or high TF diets 60 fat energy for 12 weeks. n vitro experiments were conducted on mouse macrophage cells RAW and to investigate whether elaidic trans 18 1 or oleic acid cis 18 1 would upregulate inflammatory markers. Results TLR4 mutant mice were heavier than WT mice. In both genotypes mice that received TF diet were significantly heavier than those mice that received LF diet P . TLR4 mutant mice compared to WT mice had significantly higher fasting blood glucose serum insulin insulin resistance serum leptin and serum cholesterol when they received TF diet P . No upregulation of iNOS or COX2 in response to either elaidic or oleic acid in macrophage cells was observed. Conclusions Loss of function mutation in TLR4 not only did not protect mice from TF-induced obesity hyperglycemia hyperinsulinemia and .
