Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Human MMP28 expression is unresponsive to inflammatory stimuli and does not correlate to the grade of intervertebral disc degeneration | Klawitter et al. Journal of Negative Results in BioMedicine 2011 10 9 http content 10 1 9 r M 1 JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE RESEARCH Open Access Human MMP28 expression is unresponsive to inflammatory stimuli and does not correlate to the grade of intervertebral disc degeneration Marina Klawitter1 Lilian Quero1 Alessando Bertolo2 Marco Mehr2 Jivko Stoyanov2 Andreas G Nerlich3 Juergen Klasen4 Nikolaus Aebli6 Norbert Boos1 4 5 and Karin Wuertz1 5 Abstract Background MMP28 epilysin is a recently discovered member of the MMP matrix metalloproteinase family that is amongst others expressed in osteoarthritic cartilage and intervertebral disc IVD tissue. In this study the hypothesis that increased expression of MMP28 correlates with higher grades of degeneration and is stimulated by the presence of proinflammatory molecules was tested. Gene expression levels of MMP28 were investigated in traumatic and degenerative human IVD tissue and correlated to the type of disease and the degree of degeneration Thompson grade . Quantification of MMP28 gene expression in human IVD tissue or in isolated cells after stimulation with the inflammatory mediators lipopolysaccharide LPS interleukin IL -1p tumor necrosis factor TNF -a or the histondeacetylase inhibitor trichostatin A was performed by real-time RT PCR. Results While MMP28 expression was increased in individual cases with trauma or disc degeneration there was no significant correlation between the grade of disease and MMP28 expression. Stimulation with LPS IL-1b TNF-a or trichostatin A did not alter MMP28 gene expression at any investigated time point or any concentration. Conclusions Our results demonstrate that gene expression of MMP28 in the IVD is not regulated by inflammatory mechanisms is donor-dependent and cannot be positively or negatively linked to the grade of degeneration and only weakly to the occurrence of trauma. New hypotheses and future studies are needed to find the role of MMP28 in
