Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Diagnosis of tuberculosis: the experience at a specialized diagnostic laboratory | Mashta et al. Journal of Negative Results in BioMedicine 2011 10 16 http content 10 1 16 r M 1 JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE RESEARCH Open Access Diagnosis of tuberculosis the experience at a specialized diagnostic laboratory Anita Mashta1 Pooja Mishra1 Sonia Philipose1 S Tamilzhalagan1 Hanif Mahmud2 Sangeeta Bhaskar1 and Pramod Upadhyay1 Abstract This work describes the experience at a tuberculosis clinical laboratory where relatively new TB diagnosis technologies nucleic acid detection of two target strands IS6110 and devR by PCR and microscopic observation drug susceptibility MODS were used. The LJ culture was the gold standard. This evaluation was done from August 2007 to July 2009 on 463 sputum samples of tuberculosis suspects at a specialized tuberculosis clinic in Delhi India. None of the tests we evaluated can accurately detect the presence or absence of Mycobacterium tuberculosis in all the samples and smear microscopy was found to be the most reliable assay in this study. The PCR assay could detect down to 2 pg of H37Rv DNA. Sensitivity specificity was and for smear positive n 228 and negative samples n 235 respectively. In the MODS assay sensitivity specificity of and was observed for smear positive and negative samples. Sputum smear microscopy had sensitivity of and specificity of . Introduction Despite the availability of effective and inexpensive therapy tuberculosis TB is one of the leading causes of death from an infectious disease. It is believed that the clinical management of TB is made more difficult by the lack of a simple and effective diagnostic test. Correct and timely diagnosis of TB is very important to achieve higher compliance with the treatment reduce transmission and to reduce the development of drug resistance. Along with the emergence of evidence based diagnosis approaches 1 a number of new technologies have been introduced 2 . These include light-emitting .
