Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule | Journal of Negative Results in BioMedicine BioMed Central Brief report Open Access Fusion of green fluorescent protein to the C-terminus of granulysin alters its intracellular localization in comparison to the native molecule Dennis A Hanson 1 and Steven F Ziegler2 Address department of Orthopaedics and Sports Medicine University of Washington Seattle WA 98195 USA and 2Benaroya Research Institute at Virginia Mason Seattle WA 98101 USA Email Dennis A Hanson - hansonda@ Steven F Ziegler - sziegler@ Corresponding author Published 10 September 2004 Received 20 November 2003 _k n__ - - Accepted 10 September 2004 Journal of Negative Results in BioMedicine 2004 3 2 doi 1477-5751-3-2 This article is available from http content 3 1 2 2004 Hanson and Ziegler licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The engineering of green fluorescent protein GFP fusion constructs in order to visibly tag a protein of interest has become a commonly used cell biology technique. Although caveats to this approach are obvious literature reports in which the chimeric molecule behaves differently than the native molecule are scant. This brief report describes one such case. Granulysin a small lytic and antimicrobial protein produced by cytotoxic lymphocytes traffics to the regulated secretory system and is subsequently released from cells upon proper stimulus. In an attempt to elucidate mechanisms by which it accumulates in and is released from cytolytic granules GFP was fused to the C-terminus of granulysin and expressed in an NK cell line. A control construct expressing the native protein was similarly expressed. The data demonstrate that while the fusion protein is expressed .
