Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Ex vivo development, expansion and in vivo analysis of a novel lineage of dendritic cells from hematopoietic stem cells. | Han et al. Journal of Immune Based Therapies and Vaccines 2010 8 8 http content 8 1 8 JOURNAL OF IMMUNE BASED THERAPIES AND VACCINES ORIGINAL RESEARCH Open Access Ex vivo development expansion and in vivo analysis of a novel lineage of dendritic cells from hematopoietic stem cells 1 2 11 13 4 Shuhong Han Yichen Wang Bei Wang Ekta Patel Starlyn Okada Li-Jun Yang Jan S Moreb Lung-Ji Chang1 Abstract Dendritic cells DCs play a key role in innate and adaptive immunity but the access to sufficient amount of DCs for basic and translational research has been limited. We established a novel ex vivo system to develop and expand DCs from hematopoietic stem progenitor cells HPCs . Both human and mouse HPCs were expanded first in feeder culture supplemented with c-Kit ligand KL stem cell factor steel factor or CD117 ligand Flt3 ligand fms-like tyrosine kinase 3 Flt3L FL thrombopoietin TPO IL-3 IL-6 and basic fibroblast growth factor bFGF and then in a second feeder culture ectopically expressing all above growth factors plus GM-CSF and IL-15. In the dual culture system CD34 HPCs differentiated toward DC progenitors DCPs which expanded more than five orders of magnitude. The DCPs showed myeloid DC surface phenotype with up-regulation of transcription factors and Id2 and DC-related factors homeostatic chemokine ligand 17 CCL17 and beta-chemokine receptor 6 CCR6 . Multiplex ELISA array and cDNA microarray analyses revealed that the DCPs shared some features of IL-4 and IL-15 DCs but displayed a pronounced proinflammatory phenotype. DCP-derived DCs showed antigen-uptake and immune activation functions analogous to that of the peripheral blood-derived DCs. Furthermore bone marrow HPC-derived DCP vaccines of tumor-bearing mice suppressed tumor growth in vivo. This novel approach of generating DCP-DCs which are different from known IL-4 and IL-15 DCs overcomes both quantitative and qualitative limitations in obtaining functional autologous DCs from a small .
