Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Formulation of a killed whole cell pneumococcus vaccine - effect of aluminum adjuvants on the antibody and IL-17 response. | HogenEsch et al. Journal of Immune Based Therapies and Vaccines 2011 9 5 http content 9 1 5 JOURNAL OF IMMUNE BASED THERAPIES AND VACCINES ORIGINAL RESEARCH Open Access Formulation of a killed whole cell pneumococcus vaccine - effect of aluminum adjuvants on the antibody and IL-17 response Harm HogenEsch1 Anisa Dunham1 Bethany Hansen1 Kathleen Anderson1 Jean-Francois Maisonneuve2 and Stanley L Hem3 Abstract Background Streptococcus pneumoniae causes widespread morbidity and mortality. Current vaccines contain free polysaccharides or protein-polysaccharide conjugates and do not induce protection against serotypes that are not included in the vaccines. An affordable and broadly protective vaccine is very desirable. The goal of this study was to determine the optimal formulation of a killed whole cell pneumococcal vaccine with aluminum-containing adjuvants for intramuscular injection. Methods Four aluminium-containing adjuvants were prepared with different levels of surface phosphate groups resulting in different adsorptive capacities and affinities for the vaccine antigens. Mice were immunized three times and the antigen-specific antibody titers and IL-17 responses in blood were analyzed. Results Although all adjuvants induced significantly higher antibody titers than antigen without adjuvant the vaccine containing aluminum phosphate adjuvant AP produced the highest antibody response when low doses of antigen were used. Aluminum hydroxide adjuvant AH induced an equal or better antibody response at high doses compared with AP. Vaccines formulated with AH but not with AP induced an IL-17 response. The vaccine formulated with AH was stable and retained full immunogenicity when stored at 4 C for 4 months. Conclusions Antibodies are important for protection against systemic streptococcal disease and IL-17 is critical in the prevention of nasopharyngeal colonization by S. pneumoniae in the mouse model. The formulation of the whole killed bacterial .
