Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Regulation of IκBα expression involves both NF-κB and the MAP kinase signaling pathways. | Journal of Inflammation BioMed Central Research Open Access Regulation of iKBa expression involves both NF-kB and the MAP kinase signaling pathways Ning Zhang Muhammad H Ahsan Lingyun Zhu Lidia C Sambucetti Anthony F Purchio and David B West Address Xenogen Corporation 860 Atlantic Avenue Alameda California 94501 USA Email Ning Zhang - Muhammad H Ahsan - HaroonA@ Lingyun Zhu - Lidia C Sambucetti - lsambucetti@ Anthony F Purchio - David B West - Corresponding author Published 05 October 2005 Received 24 March 2005 Journal of Inflammation 2005 2 10 doi 1476-9255-2-10 Accepted 05 October 2005 This article is available from http content 2 1 10 2005 Zhang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract IKBa is an inhibitor of the nuclear transcription factor NF-kB. Binding of IKBa to NF-kB inactivates the transcriptional activity of NF-kB. Expression of IKBa itself is regulated by NF-kB which provides auto-regulation of this signaling pathway. Here we present a mouse model for monitoring in vivo IKBa expression by imaging iKBa-luc transgenic mice for IKBa promoter driven luciferase activity. We demonstrated a rapid and systemic induction of IKBa expression in the transgenic mice following treatment with LPS. The induction was high in liver spleen lung and intestine and lower in the kidney heart and brain. The luciferase induction in the liver correlated with increased IKBa mRNA level. Pre-treatment with proteasome inhibitor bortezomib dramatically suppressed LPS-induced luciferase activity. The p38 kinase inhibitor SB203580 also showed moderate inhibition of .
