Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Proteinase-activated receptor-2: two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon. | Journal of Inflammation BioMed Central Research Proteinase-activated receptor-2 two potential inflammatory mediators of the gastrointestinal tract in Atlantic salmon Jim Thorsen Einar Lilleeng Elin Christine Valen and Ashild Krogdahl Address Aquaculture Protein Centre Basic Science and Aquatic Medicine Norwegian School of Veterinary Science Oslo Norway Email Jim Thorsen - Einar Lilleeng - Elin Christine Valen - Ashild Krogdahl - Corresponding author Open Access Published 23 October 2008 Received 18 July 2008 Journal of Inflammation 2008 5 18 doi 1476-9255-5-18 Accepted 23 October 2008 This article is available from http content 5 1 18 2008 Thorsen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ Proteinase-activated receptor 2 PAR-2 activated by trypsin and other serine proteinases is a key initiator of inflammatory responses in the intestine of mammals. Atlantic salmon fed diets with standard qualities of soybean meal SBM show enteritis of the distal intestine as well as increased activity of trypsin in both luminal contents and wall tissue. Luminal trypsin activity may possibly be involved in immune related disorders of the intestine also in Atlantic salmon via activation of PAR 2. In the present study our aim was to investigate if PAR-2 play a role in SBM induced enteritis. We performed multiple alignments based on nucleic acid sequences of PAR-2 from various animals available from public databases and designed primers for use in cloning of the Atlantic salmon PAR-2 transcript. We further cloned and .
