Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Protein Never in Mitosis Gene A Interacting-1 regulates calpain activity and the degradation of cyclooxygenase-2 in endothelial cells;. | Journal of Inflammation BioMed Central Research Protein Never in Mitosis Gene A Interacting-1 regulates calpain activity and the degradation of cyclooxygenase-2 in endothelial cells Tongzheng Liu1 Ryan A Schneider1 Vaibhav Shah1 Yongcheng Huang1 2 Rostislav I Likhotvorik1 Lakhu Keshvara1 and Dale G Hoyt 1 Open Access Address division of Pharmacology The Ohio State University College of Pharmacy and The Dorothy M. Davis Heart and Lung Research Institute Columbus Ohio 43210 USA. and 2Department of Molecular Genetics University of Texas Southwestern Medical Center at Dallas 5323 Harry Hines Blvd Dallas Texas 75390 USA. Email Tongzheng Liu - Ryan A Schneider - Vaibhav Shah - Yongcheng Huang - Rostislav I Likhotvorik - likhotvorik@ Lakhu Dale G Hoyt - Corresponding author Published 22 June 2009 Received 16 February 2009 Journal of Inflammation 2009 6 20 doi l476-9255-6-20 Accepted 22 June 2009 This article is available from http content 6 1 20 2009 Liu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The peptidyl-proline isomerase Protein Never in Mitosis Gene A Interacting-1 PIN1 regulates turnover of inducible nitric oxide synthase iNOS in murine aortic endothelial cells MAEC stimulated with E. coli endotoxin LPS and interferon-Y IFN . Degradation of iNOS was reduced by a calpain inhibitor suggesting that PIN1 may affect induction of other calpain-sensitive inflammatory proteins such as cyclooxygenase COX -2 in MAEC. Methods MAEC transduced with lentivirus encoding an inactive control short hairpin sh RNA or one
