Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C. | Journal of Inflammation BioMed Central Open Access Protein kinase C promotes restoration of calcium homeostasis to platelet activating factor-stimulated human neutrophils by inhibition of phospholipase C Gregory RTintinger 1 Annette J Theron2 Helen C Steel2 Riana Cockeran2 Lynette Pretorius2 and Ronald Anderson2 Address Department of Internal Medicine Faculty of Health Sciences University of Pretoria South Africa and 2Medical Research Council Unit for Inflammation and Immunity Department of Immunology School of Medicine Faculty of Health Sciences University of Pretoria and Tshwane Academic Division of the National Health Laboratory Service Pretoria South Africa Email Gregory RTintinger - grtintinger@ Annette J Theron - Helen C Steel - hsteel@ Riana Cockeran - Lynette Pretorius - netta705@ Ronald Anderson - randerso@ Corresponding author Published 30 October 2009 Received 26 March 2009 Journal of Inflammation 2009 6 29 doi 1476-9255-6-29 Accepted 30 October 2009 This article is available from http content 6 1 29 2009 Tintinger et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The role of protein kinase C PKC in regulating the activity of phospholipase C PLC in neutrophils activated with the chemoattractant platelet-activating factor PAF 20 and 200 nM was probed in the current study using the selective PKC inhibitors GFI0903X - I pM and staurosporine 400 nM . Methods Alterations in cytosolic Ca2 Ca2 influx inositol triphosphate IP3 and leukotriene B4 production were measured using spectrofluorimetric radiometric and competitive binding radioreceptor and immunoassay .
