Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. | Nadworny et al. Journal of Inflammation 2010 7 13 http content 7 1 13 JOURNAL OF INFLAMMATION RESEARCH Open Access Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis Patricia L Nadworny1 2 JianFei Wang3 Edward E Tredget3 Robert E Burrell1 2 Abstract Background Nanocrystalline silver dressings have anti-inflammatory activity unlike solutions containing Ag only which may be due to dissolution of multiple silver species. These dressings can only be used to treat surfaces. Thus silver-containing solutions with nanocrystalline silver properties could be valuable for treating hard-to-dress surfaces and inflammatory conditions of the lungs and bowels. This study tested nanocrystalline silver-derived solutions for anti-inflammatory activity. Methods Inflammation was induced on porcine backs using dinitrochlorobenzene. Negative and positive controls were treated with distilled water. Experimental groups were treated with solutions generated by dissolving nanocrystalline silver in distilled water adjusted to starting pHs of 4 using CO2 as is 7 and 9 using Ca OH 2 . Solution samples were analyzed for total silver. Daily imaging biopsying erythema and oedema scoring and treatments were performed for three days. Biopsies were processed for histology immunohistochemistry for IL-4 IL-8 IL-10 TNF-a EGF KGF KGF-2 and apoptotic cells and zymography MMP-2 and -9 . One-way ANOVAs with Tukey-Kramer post tests were used for statistical analyses. Results Animals treated with pH 7 and 9 solutions showed clear visual improvements. pH 9 solutions resulted in the most significant reductions in erythema and oedema scores. pH 4 and 7 solutions also reduced oedema scores. Histologically all treatment groups demonstrated enhanced re-epithelialisation with decreased inflammation. At 24 h pMMP-2 expression was significantly lowered with pH and 9 treatments as was aMMP-2 expression with pH 9 treatments. In .
