Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Tumor-infiltrating effector cells of α-galactosylceramide-induced antitumor immunity in metastatic liver tumor. | Journal of Immune Based Therapies and Vaccines Original research BioMed Central Open Access Tumor-infiltrating effector cells of a-galactosylceramide-induced antitumor immunity in metastatic liver tumor Takuya Osada 1 2 Hirokazu Nagawa1 and Yoichi Shibata2 Address Department of Surgical Oncology Graduate School of Medicine The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-8655 Japan and 2Department of Transfusion Medicine Graduate School of Medicine The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 1138655 Japan Email Takuya Osada - takuosa1962@ Hirokazu Nagawa - nagawa-1su@ Yoichi Shibata - yshibata-tky@ Corresponding author Published 13 July 2004 Received 11 May 2004 Journal of Immune Based Therapies and Vaccines 2004 2 7 doi 186 1476-8518-2-7 Accepted I 3 Jtily 2004 This article is available from http content 2 1 7 2004 Osada et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background a-Galactosylceramide a-GalCer can be presented by CD1d molecules of antigenpresenting cells and is known to induce a potent NKT cell-dependent cytotoxic response against tumor cells. However the main effector cells in a-GalCer-induced antitumor immunity are still controversial. Methods In order to elucidate the cell phenotype that plays the most important role in a-GalCer-induced antitumor immunity we purified and analyzed tumor-infiltrating leukocytes TILs from liver metastatic nodules of a colon cancer cell line Colon26 comparing a-GalCer- and control vehicle-treated mice. Flow cytometry was performed to analyze cell phenotype in TILs and IFN-Y ELISA was performed to detect antigen-specific immune response. Results Flow cytometry analysis showed a significantly higher infiltration of NK cells DX5 T cell receptor
