Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: RBCS1 expression in coffee: Coffea orthologs, Coffea arabica homeologs, and expression variability between genotypes and under drought stress | BMC Plant Biology RBCS1 expression in coffee Coffea orthologs Coffea arabica homeologs and expression variability between genotypes and under drought stress Marraccini et al. Marraccini et al. BMC Plant Biology 2011 11 85 http 1471-2229 11 85 16 May 2011 2 BioMed Central Marraccini et al. BMC Plant Biology 2011 11 85 http 1471-2229 11 85 BMC Plant Biology RESEARCH ARTICLE Open Access RBCS1 expression in coffee Coffea orthologs Coffea arabica homeologs and expression variability between genotypes and under drought stress 1 1 111 Pierre Marraccini Luciana P Freire Gabriel SC Alves Natalia G Vieira Felipe Vinecky Sonia Elbelt Humberto JO Ramos3 4 Christophe Montagnon5 Luiz GE Vieira3 Thierry Leroy2 David Pot2 Vânia A Silva6 Gustavo C Rodrigues7 and Alan C Andrade1 Abstract Background In higher plants the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal . photochemical and biochemical effects. In particular a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes which encode the Rubisco small subunit under water stress. Results Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica one carried by the C. canephora sub-genome called CaCc and the other carried by the C. eugenioides sub-genome called CaCe . Using specific primer pairs for each homeolog expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand CaCc was expressed in C. canephora but almost completely silenced in non-introgressed pure genotypes of C. arabica. However enhanced CaCc expression was observed in most C. arabica cultivars with .
