Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of tissue-specific, abiotic stressresponsive gene expression patterns in wine grape (Vitis vinifera L.) based on curation and mining of large-scale EST data sets | Tillett et al. BMC Plant Biology 2011 11 86 http 1471-2229 11 86 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of tissue-specific abiotic stress-responsive gene expression patterns in wine grape Vitis vinifera L. based on curation and mining of large-scale EST data sets 1 2 1 1 f . I-X z- 1 1 Richard L Tillett Ali Ergul Rebecca L Albion Karen A Schlauch Grant R Cramer and John C Cushman Abstract Background Abiotic stresses such as water deficit and soil salinity result in changes in physiology nutrient use and vegetative growth in vines and ultimately yield and flavor in berries of wine grape Vitis vinifera L. Large-scale expressed sequence tags ESTs were generated curated and analyzed to identify major genetic determinants responsible for stress-adaptive responses. Although roots serve as the first site of perception and or injury for many types of abiotic stress EST sequencing in root tissues of wine grape exposed to abiotic stresses has been extremely limited to date. To overcome this limitation large-scale EST sequencing was conducted from root tissues exposed to multiple abiotic stresses. Results A total of 62 236 expressed sequence tags ESTs were generated from leaf berry and root tissues from vines subjected to abiotic stresses and compared with 32 286 ESTs sequenced from 20 public cDNA libraries. Curation to correct annotation errors clustering and assembly of the berry and leaf ESTs with currently available V. vinifera full-length transcripts and ESTs yielded a total of 13 278 unique sequences with 2302 singletons and 10 976 mapped to V. vinifera gene models. Of these 739 transcripts were found to have significant differential expression in stressed leaves and berries including 250 genes not described previously as being abiotic stress responsive. In a second analysis of 16 452 ESTs from a normalized root cDNA library derived from roots exposed to multiple shortterm abiotic stresses 135 genes with root-enriched .
