Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Arice calcium-dependent protein kinase is expressed in cortical root cells during the presymbiotic phase of the arbuscular mycorrhizal symbiosis | Campos-Soriano et al. BMC Plant Biology 2011 11 90 http 1471-2229 11 90 BMC Plant Biology RESEARCH ARTICLE Open Access A rice calcium-dependent protein kinase is expressed in cortical root cells during the presymbiotic phase of the arbuscular mycorrhizal symbiosis Lidia Campos-Soriano1 Jorge Gomez-Ariza2 Paola Bonfante2 and Blanca San Segundo1 Abstract Background The arbuscular mycorrhizal AM symbiosis consists of a mutualistic relationship between soil fungi and roots of most plant species. This association provides the arbuscular mycorrhizal fungus with sugars while the fungus improves the uptake of water and mineral nutrients in the host plant. Then the establishment of the arbuscular mycorrhizal AM symbiosis requires the fine tuning of host gene expression for recognition and accommodation of the fungal symbiont. In plants calcium plays a key role as second messenger during developmental processes and responses to environmental stimuli. Even though calcium transients are known to occur in host cells during the AM symbiosis the decoding of the calcium signal and the molecular events downstream are only poorly understood. Results The expression of seventeen Calcium-dependent Protein Kinase CPK genes representative of the four distinct phylogenetic groups of rice CPKs was monitored during the presymbiotic phase of the AM symbiosis. Among them OsCPK18 and OsCPK4 were found to be transcriptionally activated in response to inoculation with the AM fungus Glomus intraradices. OsCPK18 and OsCPK4 gene expression was also up-regulated by fungal-produced diffusible molecules. Laser microdissection revealed expression of OsCPK18 in cortical cells and not in epidermal cells of G. intraradices-inoculated rice roots suggesting a preferential role of this gene in the root cortex. Moreover a plasma membrane localization of OsCPK18 was observed by transient expression assays of green fluorescent protein-tagged OsCPK18 in onion epidermal cells. We also show .
