Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin | BMC Plant Biology Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin Sánchez-Pons et al. Sánchez-Pons et al. BMC Plant Biology 2011 11 91 http 1471-2229 11 91 19 May 2011 BioMed Central Sánchez-Pons et al. BMC Plant Biology 2011 11 91 http 1471-2229 11 91 BMC Plant Biology RESEARCH ARTICLE Open Access Transcriptomic and proteomic profiling of maize embryos exposed to camptothecin Nuria Sánchez-Pons Sami Irar Nora Garcia-Muniz and Carlos M Vicient Abstract Background Camptothecin is a plant alkaloid that specifically binds topoisomerase I inhibiting its activity and inducing double stranded breaks in DNA activating the cell responses to DNA damage and in response to severe treatments triggering cell death. Results Comparative transcriptomic and proteomic analyses of maize embryos that had been exposed to camptothecin were conducted. Under the conditions used in this study camptothecin did not induce extensive degradation in the genomic DNA but induced the transcription of genes involved in DNA repair and repressed genes involved in cell division. Camptothecin also affected the accumulation of several proteins involved in the stress response and induced the activity of certain calcium-dependent nucleases. We also detected changes in the expression and accumulation of different genes and proteins involved in post-translational regulatory processes. Conclusions This study identified several genes and proteins that participate in DNA damage responses in plants. Some of them may be involved in general responses to stress but others are candidate genes for specific involvement in DNA repair. Our results open a number of new avenues for researching and improving plant resistance to DNA injury. Background Maintenance of genome stability is of critical importance for all organisms. Genomic DNA is continuously subject to many types of damage resulting from endogenous factors production of reactive oxygen
