Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Do mitochondria play a role in remodelling lace plant leaves during programmed cell death? | BMC Plant Biology Do mitochondria play a role in remodelling lace plant leaves during programmed cell death Lord et al. Lord et al. BMC Plant Biology 2011 11 102 http 1471-2229 11 102 6 June 2011 BioMed Central Lord et al. BMC Plant Biology 2011 11 102 http 1471-2229 11 102 BMC Plant Biology RESEARCH ARTICLE Open Access Do mitochondria play a role in remodelling lace plant leaves during programmed cell death Christina EN Lord Jaime N Wertman Stephanie Lane and Arunika HLAN Gunawardena Abstract Background Programmed cell death PCD is the regulated death of cells within an organism. The lace plant Aponogeton madagascariensis produces perforations in its leaves through PCD. The leaves of the plant consist of a latticework of longitudinal and transverse veins enclosing areoles. PCD occurs in the cells at the center of these areoles and progresses outwards stopping approximately five cells from the vasculature. The role of mitochondria during PCD has been recognized in animals however it has been less studied during PCD in plants. Results The following paper elucidates the role of mitochondrial dynamics during developmentally regulated PCD in vivo in A. madagascariensis. A single areole within a window stage leaf PCD is occurring was divided into three areas based on the progression of PCD cells that will not undergo PCD NPCD cells in early stages of PCD EPCD and cells in late stages of PCD LPCD . Window stage leaves were stained with the mitochondrial dye MitoTracker Red CMXRos and examined. Mitochondrial dynamics were delineated into four categories M1-M4 based on characteristics including distribution motility and membrane potential ATm . A TUNEL assay showed fragmented nDNA in a gradient over these mitochondrial stages. Chloroplasts and transvacuolar strands were also examined using live cell imaging. The possible importance of mitochondrial permeability transition pore PTP formation during PCD was indirectly examined via
