Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds | McKeown et al. BMC Plant Biology 2011 11 113 http 1471-2229 11 113 BMC Plant Biology RESEARCH ARTICLE Open Access Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds Dator r Í Ir k íom A n1 t C i2 I 2mi liollo-rii imrai 1 t Pimfi Pi inc2t Philim A mlff3 4 í loir A sr hmiH5 K larlv TA rìmnmm hl IO1 Petel C IVICKcUWII Sylvia Laouielle Duplat Pjotl Plins Philip Wolf Male W Scnmid IVialk IA Donognue Antoine Folt1 Dolota Duszynska1 Aulelie Comte1 Nga Thi Lao1 Tlevol J Wennblom6 Geelt Smant2 Claudia Kohlel3 4 Ueli Glossniklaus5 and Chalies Spillane1 Abstract Background Epigenetic regulation of gene dosage by genomic implinting of some autosomal genes facilitates nolmal reproductive development in both mammals and floweling plants. While many implinted genes have been identified and intensively studied in mammals smaller numbels have been characterized in flowering plants mostly in Arabidopsis thaliana. Identification of additional imprinted loci in flowering plants by genome-wide screening fol palent-of-oligin specific uniparental expression in seed tissues will facilitate oul undelstanding of the oligins and functions of implinted genes in floweling plants. Results cDNA-AFLP can detect allele-specific expression that is palent-of-oligin dependent fol expressed genes in which restriction site polymolphisms exist in the transcripts delived from each allele. Using a genome-wide cDNA-AFLP screen sulveying allele-specific expression of 4500 tlansclipt-delived fragments we report the identification of 52 maternally expressed genes MEGs displaying palent-of-oligin dependent expression patterns in Alabidopsis siliques containing F1 hyblid seeds 3 4 and 5 days aftel pollination . We identified these MEGs by developing a bioinfolmatics tool GenFlag which can directly detelmine the identities of tlansclipt-delived fragments flom i theil size and ii which selective nucleotides were added to the .
